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"Just add small molecules" cell-free protein synthesis: Combining DNA template and cell extract preparation into a single fermentation.
Biotechnol Prog. 2023 May-Jun;39(3):e3332. doi: 10.1002/btpr.3332. Epub 2023 Mar 5.
Biotechnol Prog. 2023.
PMID: 36799109
Hydrofoam and oxygen headspace bioreactors improve cell-free therapeutic protein production yields through enhanced oxygen transport.
Nelson JAD, Barnett RJ, Hunt JP, Foutz I, Welton M, Bundy BC.
Nelson JAD, et al.
Biotechnol Prog. 2021 Mar;37(2):e3079. doi: 10.1002/btpr.3079. Epub 2020 Oct 24.
Biotechnol Prog. 2021.
PMID: 32920987
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Rapid sensing of clinically relevant glutamine concentrations in human serum with metabolically engineered E. coli-based cell-free protein synthesis.
Hunt JP, Barnett RJ, Robinson H, Soltani M, Nelson JAD, Bundy BC.
Hunt JP, et al. Among authors: nelson jad.
J Biotechnol. 2021 Jan 10;325:389-394. doi: 10.1016/j.jbiotec.2020.09.011. Epub 2020 Sep 19.
J Biotechnol. 2021.
PMID: 32961202
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Streamlining the preparation of "endotoxin-free" ClearColi cell extract with autoinduction media for cell-free protein synthesis of the therapeutic protein crisantaspase.
Hunt JP, Zhao EL, Soltani M, Frei M, Nelson JAD, Bundy BC.
Hunt JP, et al. Among authors: nelson jad.
Synth Syst Biotechnol. 2019 Dec 13;4(4):220-224. doi: 10.1016/j.synbio.2019.11.003. eCollection 2019 Dec.
Synth Syst Biotechnol. 2019.
PMID: 31890926
Free PMC article.
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